Nanopore Sequencing: A novel technology for sequencing individual DNA molecules using single-channel recording techniques has been conceived. Single molecules of DNA are drawn through a small channel or nanopore that functions as a sensitive detector. The detection schemes being developed will transduce the different chemical and physical properties of each base into a characteristic electronic signal. Nanopore sequencing has the potential of reading very long stretches of DNA at rates exceeding 1 base per millisecond.
For a more in-depth look at this project, please go to http://mcb.harvard.edu/branton/resdes.html.
Selected Publications:
Kasianowicz, J, E. Brandin, D. Branton, and D.W. Deamer. 1996. Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. U.S.A. 93: 13770-13773.
Lue, R.A., E. Brandin, E.P. Chan, and D. Branton. 1996. Two independent domains of hDlg are sufficient for subcellular targeting: the PDZ1-2 conformational unit and an alternatively spliced domain. J. Cell Biol. 135: 1125-1137.
Lee, J.K., E. Brandin, D. Branton, and L.S.B. Goldstein. 1997. Alpha-spectrin
is required for ovarian follicle monolayer integrity in Drosophila melanogaster.
Development 124: 353:363.